Linked Life Data

5115831

Source:http://linkedlifedata.com/resource/pubmed/id/5115831

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1972-1-10
pubmed:abstractText
Hyperimmune heterologous serum produced in sheep against mouse Ehrlich ascites tumour cells was absorbed with normal mouse tissue and fractionated by DEAE column chromatography into IgG1 and IgG2 fractions. In vitro cytotoxicity test showed that sheep anti-Ehrlich ascites tumour IgGl fraction was cytotoxic to (51)Cr labelled tumour cells whereas IgG2 had no cytotoxic effect. Pretreatment of the tumour cells with the non-cytotoxic IgG2 fraction slightly inhibited the cytotoxic action of IgG1 in vitro.When EAT cells were coated with either IgG1 or IgG2 by preincubation in vitro before injecting intraperitoneally into mice, both fractions protected the animals against tumour growth. Injection of IgG2 and IgGl fractions separately, one before and the other after the injection of EAT cells, resulted in partial protection only. The difference encountered between the in vitro and in vivo findings could be attributed to the host defence mechanisms involved in the in vivo test system.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-22
pubmed:dateRevised
2011-7-20
pubmed:meshHeading
pubmed:year
1971
pubmed:articleTitle
The effect of antiserum fractions on Ehrlich ascites tumour cells.
pubmed:publicationType
Journal Article, In Vitro