Linked Life Data

50242

Source:http://linkedlifedata.com/resource/pubmed/id/50242

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1975-10-30
pubmed:abstractText
Wide variations are observed in the potency of allergen preparations available for use on humans. There are numerous methods of in vivo or in vitro testing: skin testing, passive anaphylaxis testing on animals, study of precipitins, histamine release tests on human leukocytes, radio-immunological assay test (RAST), blasticlymphocyte transformation test (TTB). The first investigations seemed to show that the allergenic potency of a large number of inducing substances was due to proteic activity; this gave rise to the theory that potency could be evaluated by protein nitrogen units (PNU). However, the protein content of an allergenic extract is not always active. In addition, natural specific proteins are altered during the extraction process or subsequently during preservation. Where the active antigen is known, comparison of different testing with various preparations may be made. The results show excellent correlation between the two biological results (cutaneous reaction and histamine release) and the content of E antigen. No relation with PNU has been observed. Certain authors propose the following: skin testing, PNU titration, dosage of certain elements (polysaccharides, lipids, phosphates, etc.), RAST. The great differences observed between the value of skin testing and the RAST may be due to the large number of active antigens in different 'dusts' and to the fact that the RAST method measures only IgE whereas allergic activity of these allergens is no doubt associated with other types of antibodies. The complexity of the problem shows that a great many studies are still necessary to provide a satisfactory solution.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5149
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
219-26
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Problems raised by potency evaluation of allergen preparations.
pubmed:publicationType
Journal Article