Linked Life Data

50006

Source:http://linkedlifedata.com/resource/pubmed/id/50006

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1975-10-10
pubmed:abstractText
A practical, simple synthesis of the obsolete mordant dye, phenocyanin, was devised, proceding from gallocyanin and resorcinol with acid and heat. The dye gave promise of good performance in metachrome iron mixtures, but because of excessive precipitation, the practice of afterchroming was taken from textile dyeing usage, and proved very successful. Of a number of metallic salts tried, Fe II proved to be the best, then Cu II and Fe III. The stain acts as a cationic dye on nucleic acids and other acidic tissue components: acid mucins, cartilage, mast cells, corpora amylacea, etc. The afterchroming process renders the stain much more resistant to various extraction agents and even moderately resistant to acid alcohol. Color values are quite comparable to those obtained with hematoxylin-eosin when an eosin counterstain is used. Nuclei basophilic cytoplasm, Nissl granules, and bacteria color dark blue; cartilage, mast cells, and some acid mucins, deep violet. Staining with the 1% solution was essentially unaltered from neutrality down to 1.2 N HCl, pH 0.68, and dilution of the dye to 0.05% in 1% conc. HCl (pH 1.2) still gave excellent nuclear, RNA, and mast-cell staining. At 0.02% and pH 1.2, nuclear staining was distinctly weakened; mast cell granules were still dark violet.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0002-9173
pubmed:author
pubmed:issnType
Print
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
876-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Hematoxylin substitutes. A study of phenocyanin TC and the use of afterchrome mordanting in histology.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.