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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1973-8-22
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pubmed:abstractText |
The recent discovery of a protein kinase activity in vaccinia virions led us to search for a viral protein which is phosphorylated in vivo. Vaccinia virus was radioactively labeled by infecting cells in the presence of (32)P(1). A phosphoprotein was isolated from purified delipidated virions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The phosphoprotein appeared to be a specific viral component induced after infection. More than 60% of the phosphoprotein was associated with viral cores. The electrophoretic mobility of the protein suggested that it has a molecular weight of 11,000 to 12,000. Phosphoserine was liberated by acid hydrolysis and identified by electrophoresis with known standards. Tryptic digests of the purified phosphoprotein were analyzed by two-dimensional electrophoresis and chromatography on thin-layer cellulose plates, and a single major phosphopeptide was resolved. The high selectivity of phosphorylation suggested that the process has a specific function.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-13909705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-14126296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-14452174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4117937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4166536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4235537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4250367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4256994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4330074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4333745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4335515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4343113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4344258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4399446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4624201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-4861258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5066568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5072434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5167067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5269234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5323798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5460142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5528242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5806584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4712965-5930347
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
961-70
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:4712965-Autoradiography,
pubmed-meshheading:4712965-Centrifugation, Density Gradient,
pubmed-meshheading:4712965-Chemical Precipitation,
pubmed-meshheading:4712965-Chromatography, DEAE-Cellulose,
pubmed-meshheading:4712965-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4712965-HeLa Cells,
pubmed-meshheading:4712965-Humans,
pubmed-meshheading:4712965-Oxidative Phosphorylation,
pubmed-meshheading:4712965-Phosphopeptides,
pubmed-meshheading:4712965-Phosphoproteins,
pubmed-meshheading:4712965-Phosphorus Isotopes,
pubmed-meshheading:4712965-Serine,
pubmed-meshheading:4712965-Threonine,
pubmed-meshheading:4712965-Tritium,
pubmed-meshheading:4712965-Trypsin,
pubmed-meshheading:4712965-Vaccinia virus,
pubmed-meshheading:4712965-Viral Proteins
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pubmed:year |
1973
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pubmed:articleTitle |
Phosphoprotein component of vaccinia virions.
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pubmed:publicationType |
Journal Article
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