Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1973-5-10
pubmed:abstractText
The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic oscillation. The antigen is largely destroyed when extracted from whole cells in 0.05 n HCl at 100 C. Acetate is recommended for routine extraction. The antigen extracted by sonic treatment was separated into six immunologically active fractions on diethylaminoethyl-Sephadex. The fractions were found to possess a common antigen which exhibited similar properties on immunodiffusion and immunoelectrophoresis. The purified antigen did not react with any other streptococcal group antisera. Adsorption of group R serum with the antigen removed all antibodies against whole cell antigen extracts of R cells. Chemical and enzymatic analysis of three fractions showed that the antigen was composed of d-glucose, d-galactose, rhamnose, and glucosamine. No significant quantities of phosphorus, glycerol, ribitol, or muramic acid were present. Significant inhibition of the quantitative precipitin determination by d-galactose and stachyose indicated that galactose in terminal alpha linkage was the immunodominant hexose in the antigen. d-Glucose and d-glucosamine possessed a partial inhibitory activity. N-acetyl-d-glucosamine and l-rhamnose did not produce significant inhibition. The results indicate that the R antigen is an immunologically specific structure which serves as a reliable means of identification of these streptococci as a serological group.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-13742188, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-13831587, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-13913775, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-14068459, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-14192543, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-14490831, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-16558032, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-16562065, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-4988040, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-5220562, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-5700279, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-5883136, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-5932578, http://linkedlifedata.com/resource/pubmed/commentcorrection/4632470-6018238
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-7
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed-meshheading:4632470-Animals, pubmed-meshheading:4632470-Antigens, Bacterial, pubmed-meshheading:4632470-Cell Wall, pubmed-meshheading:4632470-Chromatography, Ion Exchange, pubmed-meshheading:4632470-Chromatography, Thin Layer, pubmed-meshheading:4632470-Galactose, pubmed-meshheading:4632470-Glucosamine, pubmed-meshheading:4632470-Glucose, pubmed-meshheading:4632470-Hydrolysis, pubmed-meshheading:4632470-Immune Sera, pubmed-meshheading:4632470-Immunochemistry, pubmed-meshheading:4632470-Immunodiffusion, pubmed-meshheading:4632470-Immunoelectrophoresis, pubmed-meshheading:4632470-Polysaccharides, Bacterial, pubmed-meshheading:4632470-Precipitin Tests, pubmed-meshheading:4632470-Rabbits, pubmed-meshheading:4632470-Rhamnose, pubmed-meshheading:4632470-Streptococcus, pubmed-meshheading:4632470-Time Factors, pubmed-meshheading:4632470-Ultrasonics
pubmed:year
1972
pubmed:articleTitle
Immunochemistry of the streptococcal group R cell wall polysaccharide antigen.
pubmed:publicationType
Journal Article