Linked Life Data

4619012

Source:http://linkedlifedata.com/resource/pubmed/id/4619012

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1975-11-6
pubmed:abstractText
Two temperature sensitive (TS) mutants (C4 tos and D2 tos) were isolated after mutagenesis of E. coli B/SM by N-methyl-N'-nitro-N-nitrosoguanidine (NMNG), which can grow even at 42 C in high-osmotic medium supplemented by addition of sucrose, NaCl or other compounds. Neither of the mutants lyzed when transferred to low-osmotic medium after growning at the nonpermissive temperature in high osmotic medium. One of these mutants, C4 tos, grew at 42 C in a long filamentous form. When bacteria growing exponentially at 30 C were shifted to 42 C, they continued to grow at a reduced rate even in low-osmotic medium. This strain could also grow or start to grow in low-osmotic medium when supplied with a factor or factors secreted from growing bacteria of another strain. This mutant strain could grow in low-osmotic medium at 42 C when it was cultured anaerobically. The other mutant strain obtained (D2 tos) displayed normal morphology even when grown at 42 C. When it was shifted from 30 to 42 C in low-osmotic medium, increase of mass, measured as optical density, continued for a while, but viability, measured as the number of colony-formers, stopped increasing and then decreased rapidly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2324
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-59
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1974
pubmed:articleTitle
Temperature-sensitive mutants of Escherichia coli B which can grow in high-osmotic medium at the nonpermissive temperature.
pubmed:publicationType
Journal Article