rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
12
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pubmed:dateCreated |
1974-2-13
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pubmed:abstractText |
Anaerobic beta-galactoside transport in whole cells and membrane vesicles from E. coli ML 308-225 is coupled to the oxidation of alpha-glycerol-P or D-lactate with fumarate as an electron acceptor. Alternatively, anaerobic beta-galactoside transport may be coupled to the oxidation of formate utilizing nitrate as electron acceptor. Both anaerobic electron-transfer systems are induced by growth of the organisms under appropriate conditions. Components of both systems are loosely bound to the membrane, necessitating the use of a modified procedure for vesicle preparation in order to demonstrate anaerobic transport in vitro. Addition of ATP or an ATP-generating system to vesicles prepared from anaerobically-grown cells or inclusion of ATP or the ATP-generating system during preparation of vesicles does not stimulate transport. The results support the conclusion that active transport under anaerobic conditions is coupled primarily to electron flow.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4587250-14109218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4587250-14135451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4587250-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4587250-4197191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4587250-4256722,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/4587250-5327072
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Dec
|
pubmed:issn |
0027-8424
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
70
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3376-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4587250-Adenosine Triphosphate,
pubmed-meshheading:4587250-Anaerobiosis,
pubmed-meshheading:4587250-Biological Transport,
pubmed-meshheading:4587250-Carbon Radioisotopes,
pubmed-meshheading:4587250-Cell Membrane,
pubmed-meshheading:4587250-Cyanides,
pubmed-meshheading:4587250-Electron Transport,
pubmed-meshheading:4587250-Escherichia coli,
pubmed-meshheading:4587250-Formates,
pubmed-meshheading:4587250-Fumarates,
pubmed-meshheading:4587250-Galactose,
pubmed-meshheading:4587250-Glycerol,
pubmed-meshheading:4587250-Glycosides,
pubmed-meshheading:4587250-Lactose,
pubmed-meshheading:4587250-Microscopy, Electron,
pubmed-meshheading:4587250-Nitrates
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pubmed:year |
1973
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pubmed:articleTitle |
Anaerobic transport in Escherichia coli membrane vesicles.
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pubmed:publicationType |
Journal Article
|