Linked Life Data

4519628

Source:http://linkedlifedata.com/resource/pubmed/id/4519628

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1974-2-13
pubmed:abstractText
A procedure for screening several thousand clones for alterations in a specific gene product was apapplied to mutagenized cultures of Dictyostelium discoideum. Six strains were recovered which had less N-acetylglucosaminidase (EC 3.2.1.30) than the wild type. In addition, we isolated four strains in which the enzyme was temperature-sensitive. The enzyme from one of these strains had an altered substrate affinity.N-Acetylglucosaminidase is present at a low level in cells grown on bacteria and increases up to 10-fold during the aggregation stage of development. The enzyme appears to be essential to maintain migrating pseudoplasmodia of normal size, as shown by the fact that all of the mutant strains which accumulate very little N-acetyl-glucosaminidase formed only small migrating pseudoplasmodia containing less than 10% the number of cells found in the wild-type pseudoplasmodia. The small pseudoplasmodia migrated at less than a third the rate of wild-type pseudoplasmodia and ultimately formed diminutive fruiting bodies. N-Acetylglucosaminidase thus appears to be a developmental enzyme that functions during the migration stage.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3356-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1973
pubmed:articleTitle
Mutations affecting N-acetylglucosaminidase in Dictyostelium discoideum.
pubmed:publicationType
Journal Article