Linked Life Data

436282

Source:http://linkedlifedata.com/resource/pubmed/id/436282

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1979-7-16
pubmed:abstractText
Detergent must be added to the strongly acidic Kober reagent to obtain reproducible and well separated peaks in continuous flow analytical methods for oestriol in pregnancy urine. Brij 35 and other polyether detergents are not satisfactory because they or their impurities interfere in the reaction causing high blank response in colorimetric reactions and diminished fluorescence responses. In a single phase fluorometric method these detergents also caused turbidity or phase separation in the solution which passed through the fluorometer. Some anionic sulphate and sulphonate detergents were also unsatisfactory being immiscible with Kober reagent. Selected cationic and betaine detergents are shown to be possible alternatives, although one which is satisfactory in the kober reagent may be unsuitable for a dilution reagent. The betaine Empigen BB was found to be suitable for inclusion in both of these reagents.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0009-8981
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
421-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Factors in the choice of detergent in automated oestriol estimation.
pubmed:publicationType
Journal Article