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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1973-6-19
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pubmed:abstractText |
1. rRNA was isolated from rat liver at short intervals after the intraperitoneal injection of [(14)C]methyl methanesulphonate (50mg/kg) or NN-di[(14)C]methylnitrosamine (2mg/kg). These doses were chosen to minimize the effects of toxicity. 2. The following methods of hydrolysis of [(14)C]methylated rRNA were employed: enzymic digestion to nucleosides at pH8; alkaline hydrolysis and conversion into nucleosides; acid hydrolysis to bases. 3. The methylation products were analysed by chromatography on columns of Dowex-50 (H(+) form) and Dowex-50 (NH(4) (+) form). 4. With both methylating agents the principal product of methylation was 7-methylguanine. Differences were obtained, however, in the molar proportions of the minor bases 3-methylcytosine, 1-methyladenine and 7-methyladenine. Methylation at the O-6 position of guanine was a significant feature of rRNA obtained from the NN-di[(14)C]methylnitrosamine-treated animals but was not detected in rRNA after treatment with [(14)C]methyl methanesulphonate.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-14097355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-14100795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-14324822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-14343142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-16748970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-4645558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-4673570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-4929248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5145906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5339944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5366927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5435496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5488921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5625710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5651644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5656642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5665912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5722690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5791738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5809222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4349110-5809223
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenine,
http://linkedlifedata.com/resource/pubmed/chemical/Alkanes,
http://linkedlifedata.com/resource/pubmed/chemical/Alkylating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Cytosine,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrosamines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Diester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonic Acids
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
519-28
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4349110-Adenine,
pubmed-meshheading:4349110-Alkanes,
pubmed-meshheading:4349110-Alkylating Agents,
pubmed-meshheading:4349110-Animals,
pubmed-meshheading:4349110-Carbon Isotopes,
pubmed-meshheading:4349110-Chromatography, Ion Exchange,
pubmed-meshheading:4349110-Cytosine,
pubmed-meshheading:4349110-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:4349110-Guanine,
pubmed-meshheading:4349110-Hydrolysis,
pubmed-meshheading:4349110-Liver,
pubmed-meshheading:4349110-Methylation,
pubmed-meshheading:4349110-Nitrosamines,
pubmed-meshheading:4349110-Phosphoric Diester Hydrolases,
pubmed-meshheading:4349110-RNA, Ribosomal,
pubmed-meshheading:4349110-Rats,
pubmed-meshheading:4349110-Ribonucleases,
pubmed-meshheading:4349110-Sulfonic Acids
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pubmed:year |
1972
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pubmed:articleTitle |
Differences in the patterns of methylation in rat liver ribosomal ribonucleic acid after reaction in vivo with methyl methanesulphonate and NN-dimethylnitrosamine.
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pubmed:publicationType |
Journal Article
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