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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1973-1-29
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pubmed:abstractText |
The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed from a pancreatic DNase digest. The following sequence of nucleotides adjacent to the RI break made in lambda DNA was deduced from these data and from the 3'-dinucleotide sequence and nearest-neighbor analysis obtained from repair synthesis with the DNA polymerase of Rous sarcoma virus [Formula: see text] The RI endonuclease cleavage of the phosphodiester bonds (indicated by arrows) generates 5'-phosphoryls and short cohesive termini of four nucleotides, (p)A(p)A(p)T(p)T. The most striking feature of the sequence is its symmetry.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-14292165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4333538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4337614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4343968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4552763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4554112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4868368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4870862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4897066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4901831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4928352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4931680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-4949033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5312500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5312501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5323016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5325727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5476726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5684009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5832284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4343974-5969076
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenine Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Cytosine Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Endonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Diester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorus Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Polynucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Thymine Nucleotides
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3448-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4343974-Adenine Nucleotides,
pubmed-meshheading:4343974-Alkaline Phosphatase,
pubmed-meshheading:4343974-Avian Sarcoma Viruses,
pubmed-meshheading:4343974-Base Sequence,
pubmed-meshheading:4343974-Coliphages,
pubmed-meshheading:4343974-Cytosine Nucleotides,
pubmed-meshheading:4343974-DNA, Viral,
pubmed-meshheading:4343974-DNA Nucleotidyltransferases,
pubmed-meshheading:4343974-Deoxyribonucleases,
pubmed-meshheading:4343974-Drug Resistance, Microbial,
pubmed-meshheading:4343974-Endonucleases,
pubmed-meshheading:4343974-Escherichia coli,
pubmed-meshheading:4343974-Extrachromosomal Inheritance,
pubmed-meshheading:4343974-Nucleotides,
pubmed-meshheading:4343974-Oligonucleotides,
pubmed-meshheading:4343974-Phosphoric Diester Hydrolases,
pubmed-meshheading:4343974-Phosphorus Isotopes,
pubmed-meshheading:4343974-Polynucleotides,
pubmed-meshheading:4343974-Templates, Genetic,
pubmed-meshheading:4343974-Thymine Nucleotides
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pubmed:year |
1972
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pubmed:articleTitle |
DNA nucleotide sequence restricted by the RI endonuclease.
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pubmed:publicationType |
Journal Article
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