Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1966-4-9
pubmed:abstractText
Vonka, Vladimir (Baylor University College of Medicine, Houston, Tex.), and Matilda Benyesh-Melnick. Interactions of human cytomegalovirus with human fibroblasts. J. Bacteriol. 91:213-220. 1966.-Virus attachment of human cytomegalovirus to human embryo lung fibroblasts was found to be temperature-independent, from 4 to 37 C. Prolonged incubation at 4 C, however, resulted in inactivation of a high proportion of attached virus. Virus penetration seemed to be temperature-dependent, occurring at 37 C but not at 4 C. Detailed studies of the growth curve of the virus were made. Cell-associated virus preceded the appearance of virus in the fluid phase by 2 to 5 days. Complement-fixing antigen could be detected, but only when the cytopathic effect was advanced, and it was demonstrable only in the cell-associated fraction. Under methyl cellulose, decreasing the bicarbonate concentration in the overlay from 0.225 to 0.15% resulted in marked increase in plating efficiency with all strains tested. However, varying the concentration of bicarbonate from 0.3 to 0.15% in fluid medium did not influence the growth of virus.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-13350367, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-13400856, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-13456366, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-13539371, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-13859608, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-13919219, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-13949074, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14040222, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14089779, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14219928, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14233466, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14233512, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14266222, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14297547, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-14297550, http://linkedlifedata.com/resource/pubmed/commentcorrection/4285532-4285533
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-20
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1966
pubmed:articleTitle
Interactions of human cytomegalovirus with human fibroblasts.
pubmed:publicationType
Journal Article, In Vitro