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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0010453,
umls-concept:C0023516,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0037993,
umls-concept:C0039195,
umls-concept:C0079411,
umls-concept:C0205307,
umls-concept:C0205430,
umls-concept:C0439662,
umls-concept:C0871261,
umls-concept:C1533691,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
3
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pubmed:dateCreated |
1974-12-10
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pubmed:abstractText |
Mouse cytotoxic T lymphocytes (CTL) were generated in mixed leukocyte cultures (MLC) using spleen cells as responding cells and irradiated allogeneic spleen cells as stimulating cells. Cytotoxicity was assessed by a quantitative (51)Cr assay system and the relative frequency of CTL in individual cell populations was estimated from dose-response curves. Inclusion of 2-mercaptoethanol in the MLC medium resulted in a 20-40-fold increase in the relative number of CTL generated at the peak of the response. Under these culture conditions, cell-mediated cytotoxic activity was detectable in MLC populations as early as 48 h after the onset of the cultures. When spleen cells from mice immunized with allogeneic tumor cells 2-4 mo previously were cultured with irradiated spleen cells of the same alloantigenic specificity (MLC-Imm), it was found that the cell-mediated cytotoxic response was detectable earlier and reached higher levels than that observed in a primary MLC. At the peak of the response, MLC-Imm populations were observed to lyse up to 50% of the target cells within 3 h at a lymphocyte: target cell ratio of 0.3:1. Immunological and physical characterization of the effector cells generated in MLC-Imm indicated that they were medium to large-sized T lymphocytes. Altogether, these studies suggested the existence of an anamnestic cell-mediated cytotoxic response in MLC-Imm.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-14434282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4123983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4143965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4151339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4158328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4168095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4269569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4278109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4401190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4401545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4541559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4559193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4567392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4568186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4623609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4645593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4727914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4922690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4966657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-4986975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-5061825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-5101177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-5417055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-5442097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-5513502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4278108-5783244
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
140
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
703-17
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pubmed:dateRevised |
2010-6-22
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pubmed:meshHeading |
pubmed-meshheading:4278108-Animals,
pubmed-meshheading:4278108-Antigen-Antibody Reactions,
pubmed-meshheading:4278108-Chromium Radioisotopes,
pubmed-meshheading:4278108-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:4278108-Immunity, Cellular,
pubmed-meshheading:4278108-Isoantigens,
pubmed-meshheading:4278108-Kinetics,
pubmed-meshheading:4278108-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:4278108-Mercaptoethanol,
pubmed-meshheading:4278108-Mice,
pubmed-meshheading:4278108-Mice, Inbred C3H,
pubmed-meshheading:4278108-Mice, Inbred C57BL,
pubmed-meshheading:4278108-Mice, Inbred DBA,
pubmed-meshheading:4278108-Spleen,
pubmed-meshheading:4278108-T-Lymphocytes
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pubmed:year |
1974
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pubmed:articleTitle |
Generation of cytotoxic T lymphocytes in vitro. I. Response of normal and immune mouse spleen cells in mixed leukocyte cultures.
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pubmed:publicationType |
Journal Article
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