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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1967-10-14
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pubmed:abstractText |
1. A simple procedure involving repeated washings of actomyosin, extracted as the complex from myofibrils (natural actomyosin) at ionic strength less than 0.002, is described for the preparation of a desensitized actomyosin. 2. The Mg(2+)-activated adenosine triphosphatase of natural actomyosin was markedly inhibited by ethylenedioxybis(ethyleneamino)tetra-acetic acid, whereas that of the desensitized actomyosin was unaffected. 3. The activity of the Ca(2+)-activated adenosine triphosphatase of natural actomyosin was generally lower than that of the Mg(2+)-activated adenosine triphosphatase, whereas in the desensitized actomyosin the difference between the activities was considerably less. In both natural and desensitized actomyosin the adenosine-triphosphatase activities in the presence of Mg(2+) were similar. 4. The conversion of the natural into the desensitized actomyosin was accompanied by the removal of a protein fraction containing the factors responsible for the sensitivity to ethylenedioxybis(ethyleneamino)tetra-acetic acid and for modifying the Ca(2+)-activated adenosine triphosphatase. When added to a desensitized actomyosin this fraction effected a reversal to the natural form. The recombination was facilitated by increasing the ionic strength of the medium. The two factors showed different stabilities to heat and tryptic digestion.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-13093625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-13437204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-13522566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-13628558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-13909665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-14005435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-14066941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-14097720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-14179458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-14432041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-14820852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-4164877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-4220887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4226811-4224839
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1967
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pubmed:articleTitle |
The adeonosine-triphosphatase activity of desensitized actomyosin.
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pubmed:publicationType |
Journal Article,
In Vitro
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