| pubmed-article:4207247 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:4207247 | lifeskim:mentions | umls-concept:C0040669 | lld:lifeskim |
| pubmed-article:4207247 | lifeskim:mentions | umls-concept:C2267774 | lld:lifeskim |
| pubmed-article:4207247 | lifeskim:mentions | umls-concept:C0004595 | lld:lifeskim |
| pubmed-article:4207247 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:4207247 | lifeskim:mentions | umls-concept:C0332148 | lld:lifeskim |
| pubmed-article:4207247 | lifeskim:mentions | umls-concept:C1533148 | lld:lifeskim |
| pubmed-article:4207247 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:4207247 | pubmed:dateCreated | 1974-6-17 | lld:pubmed |
| pubmed-article:4207247 | pubmed:abstractText | The host bacterium of bacteriophage GA-1, Bacillus sp. G1R, was compared with respect to its taxonomic relationship to Bacillus subtilis, B. licheniformis, and B. pumilis. The physiological-biochemical properties of Bacillus sp. G1R are equal to those of B. licheniformis, but the thermal denaturation midpoint of G1R DNA differs by 3 C and the buoyant density by 0.005 g/cm(3) from that of B. licheniformis. Transformation with G1R donor DNA was neither observed in B. licheniformis nor in B. subtilis-competent recipients. Bacteriophage GA-1 shows neither infectivity on B. licheniformis nor on B. subtilis. However, infection of competent B. subtilis cultures with phenol-extracted GA-1 DNA results in the production of infective GA-1 particles. The transfecting activity of GA-1 DNA is destroyed by treatment with proteolytic enzymes. Resistance of transfecting DNA to inactivation by trypsin develops earlier than that to inactivation by DNase. Protease-treated GA-1 DNA competes with transforming DNA to approximately the same extent as does untreated GA-1 DNA, suggesting that uptake of GA-1 DNA is not affected by protease treatment. CsCl density gradient centrifugation reveals that the density of trypsinized GA-1 DNA is 0.004 g/cm(3) greater than that of untreated DNA. | lld:pubmed |
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| pubmed-article:4207247 | pubmed:language | eng | lld:pubmed |
| pubmed-article:4207247 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:4207247 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:4207247 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:4207247 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:4207247 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:4207247 | pubmed:author | pubmed-author:ArwertFF | lld:pubmed |
| pubmed-article:4207247 | pubmed:author | pubmed-author:VenemaGG | lld:pubmed |
| pubmed-article:4207247 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:4207247 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:4207247 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:4207247 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:4207247 | pubmed:pagination | 584-9 | lld:pubmed |
| pubmed-article:4207247 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
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| pubmed-article:4207247 | pubmed:year | 1974 | lld:pubmed |
| pubmed-article:4207247 | pubmed:articleTitle | Protease-sensitive transfection of Bacillus subtilis with bacteriophage GA-1 DNA: a probable case of heterologous transfection. | lld:pubmed |
| pubmed-article:4207247 | pubmed:publicationType | Journal Article | lld:pubmed |
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