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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1974-5-1
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pubmed:abstractText |
Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [(125)I]S3 polysaccharide-protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti-S3 antibodies of the donor rabbit. These observations strongly suggest that a normal committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:4150021-Animals,
pubmed-meshheading:4150021-Antibodies,
pubmed-meshheading:4150021-Antibodies, Bacterial,
pubmed-meshheading:4150021-Antibody Formation,
pubmed-meshheading:4150021-Antibody Specificity,
pubmed-meshheading:4150021-Antigen-Antibody Reactions,
pubmed-meshheading:4150021-Bacterial Vaccines,
pubmed-meshheading:4150021-Cell Transformation, Neoplastic,
pubmed-meshheading:4150021-Immunization,
pubmed-meshheading:4150021-Iodine Radioisotopes,
pubmed-meshheading:4150021-Isoelectric Focusing,
pubmed-meshheading:4150021-Rabbits,
pubmed-meshheading:4150021-Radioimmunoassay,
pubmed-meshheading:4150021-Simian virus 40,
pubmed-meshheading:4150021-Spleen,
pubmed-meshheading:4150021-Streptococcus pneumoniae
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pubmed:year |
1974
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pubmed:articleTitle |
Transformation by simian virus 40 of spleen cells from a hyperimmune rabbit: demonstration of production of specific antibody to the immunizing antigen.
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pubmed:publicationType |
Journal Article
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