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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1972-4-7
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pubmed:abstractText |
Addition of polyadenylic-polyuridylic acid in complex form (poly A:U) without antigen to a suspension of spleen cells obtained from BALB/Aj mice primed 6 wk previously with human gamma-globulin (HGG) resulted in an immediate fourfold increase over background number of anti-HGG rosette-forming cells (RFC). Culture of similar cells in the presence of puromycin for 1-6 hr before poly A:U did not significantly reduce the response. Continued culture of primed spleen cells in the presence of poly A:U, resulted in a decrease of RFC to background levels within an hour followed by an increase again 6 hr later. This later increase in RFC was inhibited by addition of puromycin to the culture medium. The nonspecific stimulation by poly A:U of antibody production by primed spleen cells also was induced in vivo. Increases in splenic RFC were detectable 6 hr after intravenous injection of poly A:U alone, without antigen, into primed mice. The response peaked at 18 hr and had dissipated completely within 3 days. A second injection of poly A:U 24 hr or later after the first injection resulted in a second response, similar to the first with respect to kinetics and intensity. Rosette formation by poly A:U-stimulated cells could not be inhibited by mitotic poisons, but was inhibited by treatment of the cells with goat anti-mouse gamma-globulin serum, suggesting that the antibody involved was a 7S gamma-globulin. The decrease in RFC induced by culture of primed cells for 1 hr in poly A:U paralleled a decrease in secondary responsiveness of the cells to antigen. This poly A:U-induced inhibition of secondary responsiveness could be reversed by suspending the treated cells in supernatant fluids derived from poly A:U-stimulated cultures. The reversal was specific in that supernatant fluids removed from bovine serum albumin (BSA)-primed cells by poly A:U did not stimulate the response of HGG-primed cells to HGG. However supernatant fluids from BSA-primed cells caused the production of anti-HGG RFC if BSA rather than HGG was used as triggering antigen. The active factor in the supernatant fluids appeared to be a 7S gamma-globulin since activity was lost after 45 min incubation of the supernatant fluids in the presence of goat anti-mouse 7S gamma-globulin serum.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-13445749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-14803635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-17842788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4099713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4102398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4106807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4159057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4176224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4187199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4189355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4192567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4192703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4860924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4867491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4927594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-4939152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5103320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5308064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5327954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5419269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5545148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5636329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5645219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5656652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5782767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5782770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5787769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5808066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/4109807-5922742
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
135
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
45-67
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:4109807-Animals,
pubmed-meshheading:4109807-Antibody Formation,
pubmed-meshheading:4109807-Cells, Cultured,
pubmed-meshheading:4109807-Chemoreceptor Cells,
pubmed-meshheading:4109807-Immunity, Cellular,
pubmed-meshheading:4109807-Immunoglobulin G,
pubmed-meshheading:4109807-Immunoglobulins,
pubmed-meshheading:4109807-Immunologic Memory,
pubmed-meshheading:4109807-Mice,
pubmed-meshheading:4109807-Mice, Inbred Strains,
pubmed-meshheading:4109807-Polynucleotides,
pubmed-meshheading:4109807-Puromycin,
pubmed-meshheading:4109807-Spleen,
pubmed-meshheading:4109807-gamma-Globulins
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pubmed:year |
1972
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pubmed:articleTitle |
Regulation of the immune system by synthetic polynucleotides. V. Effect on cell-associated immunoglobulin receptors and immunological memory.
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pubmed:publicationType |
Journal Article,
In Vitro
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