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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1986-2-17
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pubmed:abstractText |
The weakly acidic, cation-exchange resin Bio-Rex 70 was used at a low pH for chromatography of enkephalins and other peptides containing enkephalin sequences. Recoveries of various peptide standards and peptides in adrenal chromaffin cell extracts were greater than 85%. Catecholamines and other substances such as density gradient media are not retained by the resin under the conditions employed. A significant purification of enkephalins and larger peptides containing enkephalin sequences with respect to total protein was also obtained by this method. This chromatographic technique should be useful where a low-cost, preliminary purification of samples containing peptides derived from proenkephalin A is required and may be adaptable to other peptides as well.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0165-0270
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
155-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:4079460-Adrenal Medulla,
pubmed-meshheading:4079460-Animals,
pubmed-meshheading:4079460-Cation Exchange Resins,
pubmed-meshheading:4079460-Cattle,
pubmed-meshheading:4079460-Chromatography, High Pressure Liquid,
pubmed-meshheading:4079460-Chromatography, Ion Exchange,
pubmed-meshheading:4079460-Endorphins,
pubmed-meshheading:4079460-Enkephalins,
pubmed-meshheading:4079460-Ion Exchange Resins,
pubmed-meshheading:4079460-Protein Precursors,
pubmed-meshheading:4079460-Resins, Synthetic
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pubmed:articleTitle |
Purification of peptides derived from proenkephalin A on Bio-Rex 70.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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