Linked Life Data

4066680

Source:http://linkedlifedata.com/resource/pubmed/id/4066680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
1986-1-16
pubmed:abstractText
A major integral membrane glycoprotein of 63 kDa (p63), present at 500,000 copies/cell, was found on the surface of Leishmania major LEM 513 promastigotes. This protein was labeled either by surface iodination of the cells or by metabolic incorporation of [35S]methionine. Peptide maps of the proteins labeled by the two procedures were identical. Protein p63 was purified in three steps: extraction and phase separation in the nonionic detergent Triton X-114, chromatography on DEAE-cellulose, and finally chromatography on a Mono-Q column. The carbohydrate content as well as the concanavalin A receptor activity were characterized. A hydrophilic form of p63 was generated during the purification of the protein. This form was not derived by proteolysis from the amphiphilic protein found in the membrane, but may have been generated by the hydrolysis of a lipid containing myristyl residue(s) anchoring the protein in the membrane.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15504-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Identification and purification of membrane and soluble forms of the major surface protein of Leishmania promastigotes.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't