Linked Life Data

4055700

Source:http://linkedlifedata.com/resource/pubmed/id/4055700

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-12-19
pubmed:abstractText
Methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (i.e., the MAIS complex of organisms). The mycobactins from these three species showed a structure that is common to the mycobactins from all the mycobacteria examined to date. However, these mycobactins were unique in that they had more than one alkyl chain. The M. scrofulaceum mycobactins differed from other MAIS mycobactins by a shift in the position of the double bond in the R1 alkyl chain. Traces of other mycobactin types were observed in ethanol extracts of the three species, and examination of the chromatographic properties of these mycobactins showed that each species produced five mycobactin types. Each mycobactin could be subdivided further by the length of its R1 alkyl chain. No differences in the production of these novel mycobactin were observed among species. Mycobactins from three strains of Mycobacterium paratuberculosis and two wood pigeon strains of Mycobacterium avium which had lost their original growth requirements for mycobactin after repeated subculturing in laboratory growth media were examined by thin-layer chromatography and high-pressure liquid chromatography. Each organism produced a mycobactin with similar chromatographic properties to those synthesized by MAIS organisms. M. paratuberculosis NADC 18 produced at least two components in our laboratory, and nuclear magnetic resonance analysis of the major component showed this mycobactin to be identical to that produced by M. intracellulare M12. However, a sample of mycobactin J isolated by Merkal and McCullough (Curr. Microbiol. 7:333-335, 1982) from M. paratuberculosis NADC 18 was different from our isolates and appeared to correspond to a minor mycobactin component we had seen by thin-layer chromatography. No reason for this difference could be evinced. Our findings indicate that there is a close taxonomic relationship between M. paratuberculosis and the MAIS complex.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-1023808, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-14344190, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-16749098, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-4828192, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-4859342, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-4918634, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-4941561, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-5360675, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-6181183, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-621732, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-6470673, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-6826517, http://linkedlifedata.com/resource/pubmed/commentcorrection/4055700-743238
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
896-903
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Isolation, identification, and structural analysis of the mycobactins of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, and Mycobacterium paratuberculosis.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't