Linked Life Data

4050440

Source:http://linkedlifedata.com/resource/pubmed/id/4050440

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1985-11-21
pubmed:abstractText
A whole cell Staphylococcus aureus enzyme-linked immunosorbent assay (ELISA) for the detection of IgG antibodies against S. aureus has been developed. To avoid non-specific binding of IgG to protein A, the protein A-poor strains of S. aureus, E 1369 and Wood 46, were used as antigens. One-hundred and eighty serum samples from 120 patients with S. aureus endocarditis, non S. aureus endocarditis, S. aureus septicaemia and non S. aureus septicaemia were tested together with sera from 155 healthy controls. The sensitivity was similar for the E 1369 ELISA and the Wood 46 ELISA and positive test values were detected in 84.2% of patients with S. aureus endocarditis and 41.2% of patients with complicated S. aureus septicaemia. No distinction could be found between complicated and uncomplicated S. aureus septicaemia. The E 1369 ELISA was more specific showing cross reactions with sera from patients infected with other bacteria than S. aureus in 3.6%. Furthermore, the reproducibility was better for the E 1369 ELISA with a coefficient of variation at 0.054. The absence of need for purified antigens makes the whole S. aureus ELISA easy, rapid and cheap. Therefore, we suggest the whole S. aureus ELISA as a good alternative to previously reported assays using purified cell wall antigens.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0108-0180
pubmed:author
pubmed:issnType
Print
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
307-13
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Antibody response against whole Staphylococcus aureus in patients with staphylococcal septicaemia and endocarditis investigated by ELISA.
pubmed:publicationType
Journal Article, Comparative Study