| pubmed-article:3947056 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3947056 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:3947056 | lifeskim:mentions | umls-concept:C0996937 | lld:lifeskim |
| pubmed-article:3947056 | lifeskim:mentions | umls-concept:C0243808 | lld:lifeskim |
| pubmed-article:3947056 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3947056 | pubmed:dateCreated | 1986-3-12 | lld:pubmed |
| pubmed-article:3947056 | pubmed:abstractText | Analysis of a Petunia hybrida cell culture (MP4-G) resistant to 1 mM glyphosate revealed a 15- to 20-fold increased level of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in the herbicide-tolerant strain. Immunoblotting and enzyme kinetic measurements established that the increased EPSP synthase activity resulted from overproduction of a herbicide-sensitive form of the enzyme. Homogeneous enzyme preparations were obtained from the herbicide-tolerant cell line by sequential ion-exchange, hydroxyapatite, hydrophobic-interaction, and molecular sieve chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and molecular sieve chromatography established the Petunia enzyme to be a monomeric protein with Mr 49,000-55,800. Km values for phosphoenolpyruvate and shikimate 3-phosphate were about 14 and 18 microM, respectively. Glyphosate inhibited the enzyme competitively with phosphoenolpyruvate (Ki = 0.17 microM). These experiments provide further evidence that EPSP synthase is a major site of glyphosate action in plant cells. | lld:pubmed |
| pubmed-article:3947056 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3947056 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3947056 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3947056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3947056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3947056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3947056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3947056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3947056 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3947056 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3947056 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:3947056 | pubmed:issn | 0003-9861 | lld:pubmed |
| pubmed-article:3947056 | pubmed:author | pubmed-author:SchulzAA | lld:pubmed |
| pubmed-article:3947056 | pubmed:author | pubmed-author:AmrheinNN | lld:pubmed |
| pubmed-article:3947056 | pubmed:author | pubmed-author:FraleyR TRT | lld:pubmed |
| pubmed-article:3947056 | pubmed:author | pubmed-author:PorterC ACA | lld:pubmed |
| pubmed-article:3947056 | pubmed:author | pubmed-author:SteinrückenH... | lld:pubmed |
| pubmed-article:3947056 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3947056 | pubmed:volume | 244 | lld:pubmed |
| pubmed-article:3947056 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3947056 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3947056 | pubmed:pagination | 169-78 | lld:pubmed |
| pubmed-article:3947056 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:3947056 | pubmed:meshHeading | pubmed-meshheading:3947056-... | lld:pubmed |
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| pubmed-article:3947056 | pubmed:meshHeading | pubmed-meshheading:3947056-... | lld:pubmed |
| pubmed-article:3947056 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3947056 | pubmed:articleTitle | Overproduction of 5-enolpyruvylshikimate-3-phosphate synthase in a glyphosate-tolerant Petunia hybrida cell line. | lld:pubmed |
| pubmed-article:3947056 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3947056 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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