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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-3-12
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pubmed:abstractText |
Analysis of a Petunia hybrida cell culture (MP4-G) resistant to 1 mM glyphosate revealed a 15- to 20-fold increased level of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in the herbicide-tolerant strain. Immunoblotting and enzyme kinetic measurements established that the increased EPSP synthase activity resulted from overproduction of a herbicide-sensitive form of the enzyme. Homogeneous enzyme preparations were obtained from the herbicide-tolerant cell line by sequential ion-exchange, hydroxyapatite, hydrophobic-interaction, and molecular sieve chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and molecular sieve chromatography established the Petunia enzyme to be a monomeric protein with Mr 49,000-55,800. Km values for phosphoenolpyruvate and shikimate 3-phosphate were about 14 and 18 microM, respectively. Glyphosate inhibited the enzyme competitively with phosphoenolpyruvate (Ki = 0.17 microM). These experiments provide further evidence that EPSP synthase is a major site of glyphosate action in plant cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-Phosphoshikimate...,
http://linkedlifedata.com/resource/pubmed/chemical/Alkyl and Aryl Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Herbicides,
http://linkedlifedata.com/resource/pubmed/chemical/Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/glyphosate
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0003-9861
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
244
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
169-78
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3947056-3-Phosphoshikimate 1-Carboxyvinyltransferase,
pubmed-meshheading:3947056-Alkyl and Aryl Transferases,
pubmed-meshheading:3947056-Cell Line,
pubmed-meshheading:3947056-Chromatography,
pubmed-meshheading:3947056-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3947056-Gene Expression Regulation,
pubmed-meshheading:3947056-Glycine,
pubmed-meshheading:3947056-Herbicides,
pubmed-meshheading:3947056-Immunoassay,
pubmed-meshheading:3947056-Kinetics,
pubmed-meshheading:3947056-Plants,
pubmed-meshheading:3947056-Transferases
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pubmed:year |
1986
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pubmed:articleTitle |
Overproduction of 5-enolpyruvylshikimate-3-phosphate synthase in a glyphosate-tolerant Petunia hybrida cell line.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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