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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1986-3-6
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pubmed:abstractText |
Troponin C from turkey skeletal muscle has been compared with its chicken counterpart in terms of amino acid composition and fragmentation patterns and with rabbit TN-C by Ca2+ binding and conformational response to Ca2+ as monitored by CD and fluorescence. Cyanogen bromide and tryptic digestion mixtures of chicken and turkey TN-C have been separated by reversed-phase HPLC. The similarity of the elution profiles, along with the almost identical amino acid compositional data, suggest that the sequences are essentially equivalent. Both turkey and rabbit TN-C bound 2 mol Ca2+/mol protein at pH 5.3, while at pH 6.8, this figure was raised to 4 mol/mol protein. Circular dichroism and fluorescence measurements indicated that the conformations of the two proteins responded in a very similar manner to the presence of Ca2+.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Troponin,
http://linkedlifedata.com/resource/pubmed/chemical/Troponin C
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
20
|
pubmed:volume |
195
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
17-22
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3943607-Amino Acids,
pubmed-meshheading:3943607-Animals,
pubmed-meshheading:3943607-Calcium,
pubmed-meshheading:3943607-Hydrogen-Ion Concentration,
pubmed-meshheading:3943607-Peptide Fragments,
pubmed-meshheading:3943607-Protein Conformation,
pubmed-meshheading:3943607-Rabbits,
pubmed-meshheading:3943607-Spectrometry, Fluorescence,
pubmed-meshheading:3943607-Troponin,
pubmed-meshheading:3943607-Troponin C,
pubmed-meshheading:3943607-Turkeys
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pubmed:year |
1986
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pubmed:articleTitle |
Comparative calcium binding and conformational studies of turkey and rabbit skeletal troponin C.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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