Linked Life Data

3931079

Source:http://linkedlifedata.com/resource/pubmed/id/3931079

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1985-11-18
pubmed:abstractText
DL-alpha-Difluoromethylornithine (DFMO), an inhibitor of the polyamine biosynthetic enzyme ornithine decarboxylase (EC 4.1.1.17), strongly retards the growth of several species of phytopathogenic fungi in vitro. Such inhibition can be completely reversed by putrescine or spermidine, confirming the essentiality of polyamines for growth of fungal hyphae. We now show that DFMO can protect bean plants (Phaseolus vulgaris Linnaeus cv. Pinto) against infection by uredospores of the bean rust fungus, Uromyces phaseoli Linnaeus, race O. Unifoliolate leaves of 10-day-old greenhouse-grown seedlings were sprayed with 400 microliter per leaf of DFMO at various concentrations in 0.01% Tween 20 at pH 7.0 before or after inoculation with uredospores of Uromyces. After 16 hr in darkness in dew chambers to facilitate spore germination, plants were transferred to the greenhouse, arranged randomly, and examined for local lesions 7 days later. All concentrations of DFMO 0.50 mM or higher gave complete protection against the pathogen; at lower concentrations, postinoculation treatments with DFMO were generally more effective than preinoculation. The appearance of lesions on plants treated with lower concentrations of DFMO was retarded 2-6 days. DFMO also confers protection on unsprayed parts of treated plants, indicating the translocation of some protective effect from sprayed areas. DL-alpha-Difluoromethylarginine, an analogous inhibitor of arginine decarboxylase (EC 4.1.1.19), which is the rate-limiting enzyme in an alternative pathway for polyamine biosynthesis in higher plants, confers no protection even at 5 mM. This emphasizes ornithine decarboxylase as the biochemical locus of choice for the prevention of plant diseases by inhibiting polyamine metabolism.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-1108876, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-11541300, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-16662530, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-3157043, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-348678, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-348679, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-387744, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-399430, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-6393877, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-6450741, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-656104, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-6788079, http://linkedlifedata.com/resource/pubmed/commentcorrection/3931079-7040829
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6874-8
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Prevention of a plant disease by specific inhibition of fungal polyamine biosynthesis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't