Linked Life Data

3924428

Source:http://linkedlifedata.com/resource/pubmed/id/3924428

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1985-8-1
pubmed:abstractText
We determined UDP-glucuronyltransferase (UDP-GT) activities of hepatic and mammary gland microsomes of female rats with p-nitrophenol and the ring- and N-hydroxylated metabolites of N-2-fluorenylacetamide (2-FAA) and the effects of hepatic inducers of UDP-GT's on these glucuronidations. Pre-treatment of non-lactating (NL) and lactating (L) rats with beta-naphthoflavone (beta-NF) significantly increased glucuronidations, of p-nitrophenol, the phenolic metabolites of 2-FAA, especially of 5-hydroxy-2-FAA, and also of N-hydroxy-2-FAA by hepatic microsomes. Pre-treatment of L rats with beta-NF or 3-methyl-cholanthrene (3-MC) significantly increased glucuronidations of these compounds by mammary gland microsomes suggesting that both liver and mammary gland of L rats possess similar UDP-GT activities. In NL rats, UDP-GT activities of mammary microsomes toward phenols were greater than in L rats, and except for that of 5- and 7-hydroxy-2-FAA, were not inducible with beta-NF. The data obtained with L rats, the greater magnitude of stimulation of the hepatic UDP-GT of NL rats by beta-NF than by phenobarbital, and the lack of effect of the latter on UDP-GT of mammary microsomes suggested that the phenolic metabolites of 2-FAA and N-hydroxy-2-FAA share chiefly the characteristics of substrates for group 1 UDP-GT activities (i.e., those inducible with beta-NF or 3-MC). Neither inducer increased glucuronidation of 9-hydroxy-2-FAA, a relatively poor substrate for UDP-GT of mammary or hepatic microsomes. In contrast to hepatic microsomes which formed considerable amounts of the glucuronide of N-hydroxy-2-FAA, mammary gland microsomes glucuronidated this substrate to a minor extent only. This suggested that glucuronide of N-hydroxy-2-FAA may play a role in systemic, but not in local mammary tumorigenesis by N-hydroxy-2-FAA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
687-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Microsomal metabolism of the carcinogen, N-2-fluorenyl-acetamide, by the mammary gland and liver of female rats. II. Glucuronidation of ring- and N-hydroxylated metabolites of N-2-fluorenylacetamide.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.