Linked Life Data

3909796

Source:http://linkedlifedata.com/resource/pubmed/id/3909796

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-1-29
pubmed:abstractText
Immunohistochemical methods were applied to the ultrastructural localization of the amyloid P component in the EHS tumor matrix. First, the preembedding approach was used by exposing frozen sections of tumor to antiserum against the mouse amyloid P component followed by the peroxidase-antiperoxidase sequence. Second, using the postembedding approach, Lowicryl K4M sections of the tumor were exposed to antiserum against the amyloid P component and subjected to the protein A-gold procedure. In both cases, the immunostaining was restricted to structures which appeared in longitudinal section as fairly straight rods and in cross section as 7- to 10-nm pentagonal or roughly circular profiles outlining a lumen with a central dot. Since these features are characteristic of basotubules, it is concluded that the basotubules of the tumor matrix possess the antigenicity of the amyloid P component and presumably contain this substance itself. Similar experiments carried out on the thick basement membrane known as Reichert's membrane demonstrated that its basotubules also possessed amyloid P-component antigenicity. It is likely, therefore, that the amyloid P component is a constituent of basotubules.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0002-9106
pubmed:author
pubmed:issnType
Print
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
399-407
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
The basement-membrane-like matrix of the mouse EHS tumor: III. Immunodetection of the amyloid P component in basotubules.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't