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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1979-11-21
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pubmed:abstractText |
An improved plaque assay for dengue virus was developed utilizing baby hamster kidney (BHK-21) cells initially grown in shaker culture. Different media preparations were tested for uniform and fast formation of BHK-21 cell sheets. Several overlay formulas were tested to develop a rapid plaque assay in 6- and 24-well plastic plates. The best results were obtained utilizing Eagle minimal essential medium (pH 7.2 to 7.4) supplemented with 1 mg of NaHCO3 per ml and 5% newborn calf serum for the formation of cell monolayers after 8 to 24 h of incubation at 37 degrees C. Serum-free Eagle minimal essential medium supplemented with 1% methylcellulose and buffered with 10 mM N-2-hydroxyethyl piperazine-N'-2-ethanesulfonic acid (pH 7.4 to 7.6) was used as an overlay medium. This system allowed for plaque formation after 3 days of incubation of dengue type 2 virus and after 4 days for dengue type 1 and 4 viruses.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-1168348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-1237269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-13601885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-13655076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-13675766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-13987273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-14402608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-16557953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-239566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-328006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4105107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4192886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4201188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4218091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4296789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4385131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4962619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4987200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-4988567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-5000191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-5002824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-6031202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-692466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/39085-909460
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
609-14
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Development of dengue virus plaques under serum-free overlay medium.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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