Linked Life Data

390086

Source:http://linkedlifedata.com/resource/pubmed/id/390086

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1980-2-26
pubmed:abstractText
Previous work (1)(1) has shown that normal human eosinophils show a preferential capacity, in comparison with neutrophils, to bind to antibody- coated schistosomula of Schistosoma mansoni. This effect is attributable to a temperature-dependent function of the eosinophil which renders its binding stable and irreversible by aggregated gamma globulin or Staphylococcus aureus protein A. In contrast, the binding of neutrophils is readily reversible by these agents. It has now been shown that the differences observed between eosinophils and neutrophils is a property of their interaction with living schistosomula. When dead or artificially damaged schistosomula were tested, neutrophils showed a markedly enhanced capacity to adhere, in both the presence and absence of anti-chistosomular serum. Subsequent experiments were designed to test the hypothesis that the strong, stable binding of eosinophils was attributable to degranulation, with release of granule contents which would then serve as ligands to bind the cell to the organism. First, an enhanced adherence both of eosinophils and of neutrophils could be demonstrated in the presence of eosinophil major basic protein (MBP) or of protamine, a high molecular weight cation. Second, the binding of eosinophils induced by concanavalin A (Con A) was found to differ markedly from that induced by antischistosomular serum. Con A-mediated binding of eosinophils was fully reversible by alpha-methyl-mannoside, was not associated with damage to the organism, and did not lead to degranulation of the cell, as estimated by measuring the release of MBP into the culture supernate. However, induction of degranulation of concanavalin A-bound eosinophils, but not of neutrophils, with the calcium ionophore A23187 converted the reaction into one which was no longer reversible by alpha- methylmannoside and in which damage to the organism now did occur. These findings support the hypothesis that the stable binding of eosinophils is associated with degranulation, a process which may contribute to the preferential capacity of this cell to mediate antibody-dependent damage to schistosomula.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-320543, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-330760, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-336632, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-4350835, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-448088, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-4631607, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-4846413, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-564249, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-570202, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-5919625, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-595093, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-596520, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-627834, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-72146, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-748337, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-748839, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-762435, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-939048, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-942977, http://linkedlifedata.com/resource/pubmed/commentcorrection/390086-974267
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
150
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1456-71
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Interactions between human eosinophils and schistosomula of Schistosoma mansoni. II. The mechanism of irreversible eosinophil adherence.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.