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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
1985-6-13
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pubmed:databankReference | |
pubmed:abstractText |
The bovine genome contains a gene family of interferon-alpha s (bIFN-alpha) that consists of at least five distinct members. Four of the bIFN-alpha genes isolated show a high degree of homology (97% in the nucleotide sequence and 93% in amino acid sequence). The overall homology in amino acid sequence of bIFN-alpha to human, murine, and rat IFNs-alpha is approximately 60%. Yet there are amino acid clusters (positions 28-41 and 118-146) which are highly conserved throughout the mammalian evolution and in which the overall homology can be as high as 86%. Within the C terminus conserved cluster there is a sequence containing 9 amino acids completely conserved in 16 mammalian IFNs-alpha and of these, 7 are also shared with a similar domain in some bacterial toxins, implying a common functional role for these domains. One of the genes, IFN-alpha C, was expressed in Escherichia coli. The purified bacterial IFN (specific activity, 2 X 10(8) units/mg) exhibited antiviral activity on bovine cells but no detectable activity was demonstrated on human and simian cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
260
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5498-504
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3886658-Amino Acid Sequence,
pubmed-meshheading:3886658-Animals,
pubmed-meshheading:3886658-Base Sequence,
pubmed-meshheading:3886658-Cattle,
pubmed-meshheading:3886658-Cloning, Molecular,
pubmed-meshheading:3886658-Escherichia coli,
pubmed-meshheading:3886658-Gene Expression Regulation,
pubmed-meshheading:3886658-Humans,
pubmed-meshheading:3886658-Interferon Type I,
pubmed-meshheading:3886658-Mice,
pubmed-meshheading:3886658-Rats,
pubmed-meshheading:3886658-Species Specificity
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pubmed:year |
1985
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pubmed:articleTitle |
Bovine interferon alpha genes. Structure and expression.
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pubmed:publicationType |
Journal Article
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