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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1980-1-19
|
| pubmed:abstractText |
The two forms of pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) present in Escherichia coli have been purified from the same cultures and crystallized. A modified procedure for the purification of type I pyruvate kinase is described. Molecular weight, subunit structure, amino acid composition, NH2-terminal amino acid, maps of tryptic peptides and conditions for crystallization have been determined for the two forms. A comparison of these data shows that the two forms are different proteins, each being a tetramer of identical subunits.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
11
|
| pubmed:volume |
570
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
248-58
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:387087-Amino Acids,
pubmed-meshheading:387087-Crystallization,
pubmed-meshheading:387087-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:387087-Escherichia coli,
pubmed-meshheading:387087-Macromolecular Substances,
pubmed-meshheading:387087-Molecular Weight,
pubmed-meshheading:387087-Pyruvate Kinase,
pubmed-meshheading:387087-Trypsin
|
| pubmed:year |
1979
|
| pubmed:articleTitle |
Two forms of pyruvate kinase in Escherichia coli. A comparison of chemical and molecular properties.
|
| pubmed:publicationType |
Journal Article
|