Linked Life Data

3857409

Source:http://linkedlifedata.com/resource/pubmed/id/3857409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-6-6
pubmed:abstractText
Three sublines of the human promyelocytic leukaemia cell line HL60 have been isolated. Histochemical, karyotypic and immunological analyses have confirmed the identity of the three sublines as HL60. However, the extent to which c-myc homologous DNA sequences are amplified in the genomes of these cells varies from 4- to 30-fold. Moreover, the relative abundance of c-myc-related RNA sequences in uninduced cells of each subline is directly proportional to the gene copy number. It is concluded that, while high levels of the c-myc gene and its transcripts may have had a role in the establishment of HL60 cells, these are not required for maintenance of the HL60 phenotype. However we cannot rule out the possibility that a low level of amplification and enhancement of transcription has a role in maintenance of this phenotype.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0145-2126
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
239-47
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Variation in myc gene amplification and expression in sublines of HL60 cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't