Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-3-12
pubmed:abstractText
Cholesteryl ester (CE) accumulation in arterial wall macrophages (foam cells) is a prominent feature of atherosclerotic lesions. We have previously shown that murine J774 macrophages, unlike mouse peritoneal macrophages, accumulate large amounts of CE from unmodified low density lipoprotein (LDL). We now report a direct comparison of acyl coenzyme A:cholesterol acyl transferase (ACAT) activity in J774 and mouse peritoneal macrophages. Despite similar chloroquine-inhibitable 125I-LDL degradation in the two macrophages, ACAT activity in LDL-treated J774 macrophages was 10-30-fold higher than that in LDL-treated mouse peritoneal macrophages. In contrast, acetyl-LDL (matched for degradation with LDL) caused marked stimulation of ACAT activity in mouse peritoneal macrophages. From these data we conclude that in the presence of LDL, J774 macrophages have a highly active ACAT cholesterol esterification pathway compared with mouse peritoneal macrophages; and in mouse peritoneal macrophages, there is a marked difference in the ability of acetyl-LDL vs. LDL to stimulate ACAT even when the lipoproteins are matched for degradation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-1126942, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-13641241, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-13671378, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-14861228, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-197883, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-218198, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-3855559, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-3905794, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-3949765, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-3958185, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-455458, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6197090, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6243325, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6254083, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6255257, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6260883, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6311077, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6321901, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6466191, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6587396, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6746661, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6772035, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-6852041, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-7234961, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-7381331, http://linkedlifedata.com/resource/pubmed/commentcorrection/3805276-7462813
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
418-26
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Foam cell-forming J774 macrophages have markedly elevated acyl coenzyme A:cholesterol acyl transferase activity compared with mouse peritoneal macrophages in the presence of low density lipoprotein (LDL) despite similar LDL receptor activity.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't