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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1976-6-2
|
| pubmed:abstractText |
Isolated protoplasmic droplets of the alga Nitella were investigated with microelectrodes under current clamp conditions. The following observations were made: 1. Long pulses of either polarity yielded almost symmetric current-voltage relations. Near the rather small resting potential (inside negative) the measuring points lay on a straight line corresponding to an apparent surface membrane resistance of 1.7 +/- 0.45 komega (mean +/- S.E.M., n=5). 2. Experiments with various pulse programs revealed no mechanism comparable to Na inactivation but stressed the electrical symmetry of the droplet with respect to the resting potential. 3. Changes of the [Ca2+] in the bathing medium between 0 to 10 mM as well as of the pH between 5 and 9 did not influence the responses. Replacing K+ by Na+ (or vice versa) or exchanging NO3 for acetylglycine or Cl- was also ineffective. These observations are not consistent with a normal excitable surface membrane. Similar responses are obtained with a RC network which is described and which may have its substrate in histological peculiarities of the protoplasmic droplet.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0031-6768
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
361
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
263-7
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
1976
|
| pubmed:articleTitle |
Electrical responses of isolated Nitella protoplasm--excitations or artifacts?
|
| pubmed:publicationType |
Journal Article
|