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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1986-11-21
|
| pubmed:abstractText |
A highly sensitive high-performance liquid chromatographic assay using amperometric detection has been developed for measuring alpha-tocopherol in plasma. Other minor components of vitamin E (beta-, gamma- and delta-tocopherols) can also be detected by this method. All-trans-retinol is simultaneously monitored by UV detection at 313 nm. This assay requires only 50 microliter of plasma for quantification of both vitamin E and A. The minimum detectable quantities for both alpha-tocopherol and retinol are 1.0 ng with corresponding signal-to-noise ratios of 4.0 and 3.5. The total chromatographic analysis time for each sample is less than 15 min. This assay has been used to determine the concentrations of vitamin A and E in plasma obtained from patients with liver disease, liver transplant patients and normal healthy volunteers.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0021-9673
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
380
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
331-8
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1986
|
| pubmed:articleTitle |
Sensitive high-performance liquid chromatographic analysis of plasma vitamin E and vitamin A using amperometric and ultraviolet detection.
|
| pubmed:publicationType |
Journal Article
|