Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-5-28
pubmed:abstractText
Exohemagglutinin was found in the culture medium of Bacteroides gingivalis 381. Exohemagglutinin was purified 3,150-fold from culture fluid by ultracentrifugation followed by gel filtration on Sepharose CL-4B and by affinity chromatography on arginine-agarose. Examination of the final preparation of exohemagglutinin by biochemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the isolated exohemagglutinin contained three major proteins but not a detectable lipopolysaccharide. Hemagglutination inhibition experiments showed that the activity of exohemagglutinin was inhibited by L-arginine and the arginine-containing peptides, although the activity was unaffected by the sugars tested. Some protein and glycoproteins that were examined also exhibited the inhibitory activity. When the bovine submaxillary mucin was chemically modified by beta-elimination and bovine serum albumin was modified by guanidination, the inhibitory effects on hemagglutination were significantly enhanced. These results suggest that the hemagglutination of the isolated exohemagglutinin may be involved in arginine residues as components of ligand-binding sites on erythrocytes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-24002, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-4115981, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-4527823, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-468376, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-528053, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-5871826, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-5958830, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6109561, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6150029, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6396347, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6573474, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6597305, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6746097, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6751304, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6885158, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6932503, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-6995428, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-7118829, http://linkedlifedata.com/resource/pubmed/commentcorrection/3699890-7216420
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
421-7
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Isolation and some properties of exohemagglutinin from the culture medium of Bacteroides gingivalis 381.
pubmed:publicationType
Journal Article