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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1988-2-3
|
| pubmed:abstractText |
A mixture of extracellular carrageenases was isolated from the cell-free medium of a culture of marine Cytophaga sp. 1k-C783 grown on ZoBell 2216 E broth with 0.1% commercial carrageenan. A single active peak of kappa-carrageenase was separated and purified from the mixture by ammonium sulfate precipitation, ion-exchange chromatography, and Sephadex G-200 gel filtration chromatography. Molecular weight of the purified kappa-carrageenase was estimated as 100,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified kappa-carrageenase had pH optimum 7.6 and temperature optimum 25 C.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0385-5600
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
31
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
869-77
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3696007-Bacteria,
pubmed-meshheading:3696007-Bacterial Proteins,
pubmed-meshheading:3696007-Chromatography,
pubmed-meshheading:3696007-Glycoside Hydrolases,
pubmed-meshheading:3696007-Hydrogen-Ion Concentration,
pubmed-meshheading:3696007-Molecular Weight,
pubmed-meshheading:3696007-Seawater,
pubmed-meshheading:3696007-Temperature,
pubmed-meshheading:3696007-Water Microbiology
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Purification of a kappa-carrageenase from marine Cytophaga species.
|
| pubmed:affiliation |
Department of Bacteriology, Faculty of Medicine, Kagoshima University.
|
| pubmed:publicationType |
Journal Article
|