3684595

Source:http://linkedlifedata.com/resource/pubmed/id/3684595

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0008633, umls-concept:C0009013, umls-concept:C0017428, umls-concept:C0205225, umls-concept:C0242772, umls-concept:C0439064, umls-concept:C0439831, umls-concept:C1516050
pubmed:issue	22
pubmed:dateCreated	1988-1-19
pubmed:abstractText	In order to rapidly assign relatively large numbers of tomato genomic clones to specific chromosomes, we have developed the following approach: groups of five to eight clones from a single copy tomato library are pooled, nick-translated, and utilized as probes against Southern blots consisting of a panel of trisomic DNAs. Since the trisomic DNAs are digested with the same enzyme used to produce the genomic library (PstI), each hybridizing band can be related to a specific genomic clone and the relative intensities of the bands can be used to assign each to a specific chromosome. With this technique, we have assigned 52 clones to specific chromosomes and verified the assignment of 21 out of 23 by genetic mapping in a segregating F2 population. In addition to selecting clones according to chromosome, the idea of using multiple clones or "molecular darts" may have broader applications, such as screening for differences between the genomes of nearly isogenic lines.
pubmed:commentsCorrections	http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-16593745, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-17246322, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-17247510, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-3026644, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-4047940, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-6089346, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-6247908, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-6456416, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-6982420, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-7433111, http://linkedlifedata.com/resource/pubmed/commentcorrection/3684595-881736
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0411011
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Nov
pubmed:issn	0305-1048
pubmed:author	pubmed-author:MillerJ CJC, pubmed-author:TanksleyS DSD, pubmed-author:YoungN DND
pubmed:issnType	Print
pubmed:day	25
pubmed:volume	15
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	9339-48
pubmed:dateRevised	2010-9-13
pubmed:meshHeading	pubmed-meshheading:3684595-Chromosome Mapping, pubmed-meshheading:3684595-Cloning, Molecular, pubmed-meshheading:3684595-Genes, pubmed-meshheading:3684595-Plants, pubmed-meshheading:3684595-Trisomy
pubmed:year	1987
pubmed:articleTitle	Rapid chromosomal assignment of multiple genomic clones in tomato using primary trisomics.
pubmed:affiliation	Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14850.
pubmed:publicationType	Journal Article, Research Support, U.S. Gov't, Non-P.H.S.