Linked Life Data

3667630

Source:http://linkedlifedata.com/resource/pubmed/id/3667630

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
31
pubmed:dateCreated
1987-12-10
pubmed:abstractText
We have previously reported the cloning of cDNAs coding for two isoforms (termed C alpha and C beta) of the catalytic (C) subunit of cAMP-dependent protein kinase (Uhler, M., Chrivia, J., and McKnight, G. S. (1986) J. Biol. Chem. 261, 15360-15363). We have constructed cDNA expression vectors for the C alpha and C beta proteins using the mouse metallothionein promoter so that mRNA transcripts from the expression vectors are inducible with Zn. In stable transformants of NIH 3T3 and AtT-20 cells, the induction of both C alpha and C beta mRNA with Zn increases the cAMP-dependent as well as the cAMP-independent kinase activity. The C alpha and C beta proteins synthesized from these expression vectors can be detected by Western blot analysis and have slightly different molecular weights as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Interestingly, the levels of RI protein are also shown to increase in cells expressing high levels of the C alpha or C beta subunits although there is no change in RI mRNA. In contrast, the levels of RII protein are not significantly affected by increasing either C alpha or C beta expression in these cell lines. We conclude that cells can compensate for the increased levels of either C alpha or C beta subunits with a corresponding elevation of RI protein implying that both isoforms of C can interact with RI to form a holoenzyme. The relevance of these experimental results to the coordinate regulation of cAMP-dependent protein kinase subunits is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
262
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15202-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Expression of cDNAs for two isoforms of the catalytic subunit of cAMP-dependent protein kinase.
pubmed:affiliation
Department of Pharmacology, University of Washington, Seattle 98195.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.