Linked Life Data

3661724

Source:http://linkedlifedata.com/resource/pubmed/id/3661724

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4 Pt 2
pubmed:dateCreated
1987-11-19
pubmed:abstractText
Microelectrode methods were used to assess the Na-dependent effects of mineralocorticoids on the conductive transport properties of cortical collecting ducts (CCD) from adrenalectomized (ADX) rabbits. Rabbits were divided into four groups: control, 3 h treatment with deoxycorticosterone (DOCA), 18 h DOCA treatment, and 18 h DOCA plus amiloride treatment. After only 3 h of DOCA treatment the transepithelial potential (VT) increased significantly from -1.4 to -8.7 mV, and after 18 h VT increased further to -19.3 mV. The basolateral membrane potential (Vb) significantly increased after 18 h (from -61.6 to -87.6 mV). However, compared with treatment with DOCA alone for 18 h, both -VT and -Vb were significantly attenuated with DOCA plus amiloride treatment to -8.3 and -72.9 mV, respectively. The apical membrane Na and K currents (INaa and IKa) and conductances (GNaa and GKa) were all increased after 3 h DOCA treatment. After 18 h of DOCA treatment, IKa, INaa, and GKa increased further, but GNaa remained unchanged. On simultaneous treatment with DOCA and amiloride, the maximum pump current (Ipmax), IKa, INaa, and GKa but not GNaa were all reduced significantly. These results support a mechanism whereby mineralocorticoids first increase GNaa. A rise in cell Na results then in the simultaneous increase in GKa and sodium transport, probably by insertion of new Na pumps. An additional direct effect of DOCA on GKa and Ipmax is not excluded.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
253
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F753-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Na-dependent effects of DOCA on cellular transport properties of CCDs from ADX rabbits.
pubmed:affiliation
Department of Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't