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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5-6
|
pubmed:dateCreated |
1987-8-28
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pubmed:abstractText |
A composite map representing a single ribosomal DNA repeat unit of the house cricket, Acheta domesticus, was constructed from overlapping cloned fragments. Sites in the repeat unit for nine restriction enzymes were mapped. R-loop mapping of sequences coding for 18 S and 28 S RNA demonstrates that the 58-kb ribosomal DNA repeat unit contains a novel-sized internal transcribed spacer of 8.4 kb. The existence of this large spacer was confirmed in genomic DNA, most if not all of the genomic repeat units containing such a spacer. A 15- to 17-kb ribosomal RNA precursor transcript is synthesized as predicted on the basis of the size of the internal transcribed spacer. The 5.8 S RNA gene is localized to a 1-kb sequence immediately 5' to the 28 S gene. The coding regions examined contain no intervening sequences analogous to those described within ribosomal DNA of other eukaryotes. Only 11% of the repeat unit codes for mature ribosomal RNA, while the remainder is nontranscribed (71%) and transcribed (18%) spacer DNA.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-2928
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
345-58
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3619881-Animals,
pubmed-meshheading:3619881-Cloning, Molecular,
pubmed-meshheading:3619881-DNA, Ribosomal,
pubmed-meshheading:3619881-Genes,
pubmed-meshheading:3619881-Gryllidae,
pubmed-meshheading:3619881-Nucleic Acid Hybridization,
pubmed-meshheading:3619881-Orthoptera,
pubmed-meshheading:3619881-RNA, Ribosomal,
pubmed-meshheading:3619881-Transcription, Genetic
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pubmed:year |
1987
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pubmed:articleTitle |
The ribosomal DNA transcription unit of the house cricket, Acheta domesticus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|