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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0005767,
umls-concept:C0014441,
umls-concept:C0030351,
umls-concept:C0045010,
umls-concept:C0180860,
umls-concept:C0329155,
umls-concept:C0581406,
umls-concept:C1512080,
umls-concept:C1522664,
umls-concept:C1546637,
umls-concept:C1550638,
umls-concept:C1704449,
umls-concept:C1704684,
umls-concept:C2827485
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pubmed:issue |
6
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pubmed:dateCreated |
1987-7-27
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pubmed:abstractText |
In this rapid, cost-effective, enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone (17-OHP) eluted from dried blood spotted on filter paper, second antibody is coated onto the microwell plate and horseradish peroxidase (EC 1.11.1.7) is the label enzyme. Antiserum to 17-OHP was prepared by using 4-(2-carboxymethylthio)-17-OHP-bovine serum albumin conjugate as immunogen. Enzyme conjugate was prepared from 4-(2-carboxymethylthio)-17-OHP and peroxidase. The blood spots are assayed in the microwells without extraction or centrifugation steps. The detection limit of the assay is 1 microgram/L, equivalent to 3.5 pg (10.6 fmol) per disc. Intra- and interassay CVs at two steroid concentrations (7.38 and 22.79 micrograms/L) ranged from 3.74 to 11.90% (n = 5), and 9.49 and 9.83% (n = 5), respectively. Results correlated well (r = 0.91) with those of a fluorescence enzyme immunoassay. The sensitivity, specificity, and precision of this method make it potentially useful in the mass screening of neonates for congenital adrenal hyperplasia.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0009-9147
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
761-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3594810-17-alpha-Hydroxyprogesterone,
pubmed-meshheading:3594810-Adrenal Hyperplasia, Congenital,
pubmed-meshheading:3594810-Antibody Specificity,
pubmed-meshheading:3594810-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3594810-Filtration,
pubmed-meshheading:3594810-Humans,
pubmed-meshheading:3594810-Hydroxyprogesterones,
pubmed-meshheading:3594810-Mass Screening
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pubmed:year |
1987
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pubmed:articleTitle |
Enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone in dried blood spotted on filter paper.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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