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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1987-6-10
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pubmed:abstractText |
Gene-sequencing studies have shown that the glucagon precursor contains two additional glucagon-like sequences, the so-called glucagon-like peptides 1 and 2 (GLP-1 and GLP-2). We developed radio-immunoassays against synthetic peptides corresponding to these sequences. Antisera were raised in rabbits after carbodiimide conjugation of peptides to BSA. The selected antisera showed neither mutual cross-reactivity nor cross-reacted with any other peptide of the glucagon family. Trypsin digestion experiments showed that both antisera were directed against the C-terminus of the antigen peptides. Labelling was performed with chloramine T, and separation with plasma-coated charcoal. The detection limit was 7 and 25 pmol/l for GLP-1 and GLP-2. Accurate measurement of both peptides in plasma required extraction. Optimum recovery was obtained with ethanol at 75% (final concentration). The concentrations measured in fasting plasma from 10 normal subjects were 107 +/- 7 pmol/l and 151 +/- 14 pmol/l for GLP-1 and GLP-2, respectively. After a mixed meal the concentrations rose slowly for 2 h reaching 145 +/- 13 and 225 +/- 15 pmol/l.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0036-5513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
165-74
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3576119-Animals,
pubmed-meshheading:3576119-Chromatography, Gel,
pubmed-meshheading:3576119-Glucagon-Like Peptide 1,
pubmed-meshheading:3576119-Glucagon-Like Peptide 2,
pubmed-meshheading:3576119-Humans,
pubmed-meshheading:3576119-Hydrogen-Ion Concentration,
pubmed-meshheading:3576119-Immune Sera,
pubmed-meshheading:3576119-Peptides,
pubmed-meshheading:3576119-Rabbits,
pubmed-meshheading:3576119-Radioimmunoassay
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pubmed:year |
1987
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pubmed:articleTitle |
Radio-immunoassays for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2).
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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