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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
8 Suppl
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pubmed:dateCreated |
1986-9-17
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pubmed:abstractText |
An estrogen receptor enzyme immunoassay kit (ER-EIA) has been evaluated in 70 human breast carcinomas against a routine cytoplasmic [3H]estradiol binding assay (ERU). A linear correlation between the ER-EIA and the ERU was observed for binding values up to 400 fmol/mg of cytosol protein. Above this value, the ERU underestimates the concentration of receptor. The ERU gave a lower number of estrogen receptor-positive tumors (50 of 70) than did the ER-EIA assay (59 of 70). In the ERU-negative ER-EIA-positive tumors, receptor values as determined by the ER-EIA assay all fell below 50 fmol/mg of protein (mean, 19.9 +/- 4.2 fmol/mg of protein). Application of an exchange procedure which estimates the total steroid binding capacity of the cytosol gave positive results in 7 of 9 ERU-negative ER-EIA-positive tumors (mean, 16.9 +/- 2.95 fmol/mg of protein). Subdivision of the binding data according to the menopausal status of the patient indicates low receptor values in premenopausal women by each assay. A correlation between the ER-EIA assay and the histological grade of tumors was observed; Grade I well-differentiated tumors were all positive, while Grade II and III tumors were 86% and 75% positive, respectively. No correlation between the ER-EIA assay and tumor lymph node stage or tumor size was observed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0008-5472
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4299s-4302s
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3524811-Adult,
pubmed-meshheading:3524811-Aged,
pubmed-meshheading:3524811-Breast Neoplasms,
pubmed-meshheading:3524811-Cytosol,
pubmed-meshheading:3524811-Female,
pubmed-meshheading:3524811-Humans,
pubmed-meshheading:3524811-Immunoenzyme Techniques,
pubmed-meshheading:3524811-Middle Aged,
pubmed-meshheading:3524811-Radioligand Assay,
pubmed-meshheading:3524811-Receptors, Estrogen
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pubmed:year |
1986
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pubmed:articleTitle |
Evaluation of an enzyme immunoassay for estrogen receptors in human breast cancers.
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pubmed:publicationType |
Journal Article
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