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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1986-6-23
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pubmed:abstractText |
Two cis-acting elements, the enhancer and the promoter, independently contribute to the cell-specific expression of the rat insulin 1 gene. The activities of these elements are presumably mediated by trans-acting factors. We have performed intracellular competition experiments that suggest the presence of a negative factor(s) that represses the enhancer activity in cells that do not express the insulin gene. In these experiments fibroblast cells (COS-7) were transfected with two plasmids: a test plasmid containing the gene for chloramphenicol acetyltransferase under the control of the thymidine kinase promoter and the insulin enhancer; and a competitor plasmid containing insulin enhancer sequences and the simian virus 40 origin of replication to permit its replication in the recipient cells. The presence of the competitor plasmid led to a 5- to 6-fold increase in chloramphenicol acetyltransferase activity as compared with the activity detected when insulin enhancer was absent from either the competitor or the test plasmid. A 5-fold increase in chloramphenicol acetyltransferase activity was also seen when the rat amylase enhancer was present on the competitor plasmid; in contrast the simian virus 40 enhancer exerted no effect. Efficient derepression required additional sequences downstream from those essential for enhancer activity. We propose that the activity of the rat insulin 1 enhancer is modulated by a negative trans-acting factor(s) that is active in cells not expressing insulin but is overridden by the dominant positive trans-acting factor(s) present in insulin-producing cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-2414846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-2424007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-286319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-2982105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-2982503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-2992802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-2996885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-325648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-3904002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-3917574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-3917575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-3921262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-3924411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-4053184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-4291934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6095113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6147198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6248237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6270673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6286831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6294651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6313211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6317184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6319021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6327064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6329749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6358900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6409417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6409418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3517853-6960240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3180-4
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3517853-Animals,
pubmed-meshheading:3517853-Cloning, Molecular,
pubmed-meshheading:3517853-DNA Replication,
pubmed-meshheading:3517853-Enhancer Elements, Genetic,
pubmed-meshheading:3517853-Gene Expression Regulation,
pubmed-meshheading:3517853-Genes, Regulator,
pubmed-meshheading:3517853-Insulin,
pubmed-meshheading:3517853-Islets of Langerhans,
pubmed-meshheading:3517853-Rats,
pubmed-meshheading:3517853-Tissue Distribution,
pubmed-meshheading:3517853-Transcription, Genetic,
pubmed-meshheading:3517853-Transcription Factors,
pubmed-meshheading:3517853-Transfection
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pubmed:year |
1986
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pubmed:articleTitle |
Regulation of rat insulin 1 gene expression: evidence for negative regulation in nonpancreatic cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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