Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1978-9-1
pubmed:abstractText
A strategy for the proteolytic fragmentation of human IgM has been developed. This method is called "cold pepsin digestion" because of its unique feature of achieving restricted peptic cleavages at 4 degrees and pH 4.0. Cold pepsin digestion has been applied successfully to produce an Fv fragment from 14 human IgM proteins. The Fv fragment consists of the heavy chain variable domain (VH) and the light chain variable domain (VL) held together by strong noncovalent interaction. Thus, each Fv fragment contains one intact antigen-binding site and represents the minimal active fragment derivable from an antibody molecule. A series of other structurally and functionally important fragments were also isolated and characterized. Two basic digestion pathways were recognized; these mainly reflect the relative accessibility of five sets of major interdomain cleavage sites.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-164506, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-190543, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-232031, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4096, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4183097, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4205527, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4208843, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4326772, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4560694, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4569769, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4626656, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4742735, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4750258, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-4984234, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-53788, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-5447531, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-5806584, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-8089, http://linkedlifedata.com/resource/pubmed/commentcorrection/351613-886186
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2649-53
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Cold pepsin digestion: a novel method to produce the Fv fragment from human immunoglobulin M.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.