Linked Life Data

3514749

Source:http://linkedlifedata.com/resource/pubmed/id/3514749

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1986-5-2
pubmed:abstractText
Chemical and functional properties of IgA binding factor(s) (IgA-BF) from both murine Con A-activated spleen cells and Fc gamma R+, Fc alpha R+ T hybridoma cells (T2D4) were studied. IgA-BF produced from the cells after preculture with IgA were purified with IgA-Sepharose. Purified IgA-BF inhibited the binding of IgA to Fc alpha R+ L5178Y T lymphoma cells, and class-specifically suppressed in vitro IgA synthesis of the pokeweed mitogen (PWM)-stimulated murine spleen cells. Both IgA-specific suppressive activity and IgA binding activity of the factor(s) were co-fractionated between BSA and OVA in gel filtration analysis. SDS-PAGE analysis of IgA-BF biosynthetically labeled with [35S]methionine showed a specific band on 56,000. Suppressive activity of IgA-BF was absorbed with lentil-lectin-Sepharose and was eluted with 0.2 M alpha-methyl-D-mannoside. The suppressive activity obtained from T2D4 cells (H-2k) and BALB/c Con A blasts (H-2d) was absorbed with the corresponding anti-H-2 and anti-I-A column and recovered in the acid-eluate. The activity was not absorbed with the unrelated anti-H-2 column. Despite the presence of MHC products, IgA-BF from both cell sources equally suppressed IgA-specific responses of BALB/c (H-2d), C3H/He (H-2k), and C57BL/10 (H-2b) spleen cells. They also suppressed IgA production as well as IgA synthesis of PWM-stimulated culture of human peripheral blood lymphocytes without affecting IgM and IgG responses. Suppression of murine and human IgA responses both in mouse and human were mediated by the molecules having the same Ia products, suggesting that there is no MHC, as well as species restriction, for the interaction between IgA-BF and their target cells. IgA-specific suppressive activity was absorbed with human B blastoid cells bearing surface IgA (Dakiki) but not with those bearing surface IgG (CESS) or murine and human T cell line cells (BW5147, L5178Y, HPB-ALL, and MOLT4), indicating that IgA-BF interact with B cells bearing IgA to suppress their differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2910-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:3514749-Animals, pubmed-meshheading:3514749-B-Lymphocytes, pubmed-meshheading:3514749-Cell Line, pubmed-meshheading:3514749-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:3514749-H-2 Antigens, pubmed-meshheading:3514749-Hemolytic Plaque Technique, pubmed-meshheading:3514749-Immunoglobulin A, pubmed-meshheading:3514749-Immunosorbent Techniques, pubmed-meshheading:3514749-Lymphokines, pubmed-meshheading:3514749-Methionine, pubmed-meshheading:3514749-Mice, pubmed-meshheading:3514749-Mice, Inbred BALB C, pubmed-meshheading:3514749-Mice, Inbred C3H, pubmed-meshheading:3514749-Mice, Inbred C57BL, pubmed-meshheading:3514749-Molecular Weight, pubmed-meshheading:3514749-Oligosaccharides, pubmed-meshheading:3514749-Prostatic Secretory Proteins, pubmed-meshheading:3514749-Receptors, Antigen, B-Cell, pubmed-meshheading:3514749-Species Specificity, pubmed-meshheading:3514749-Suppressor Factors, Immunologic
pubmed:year
1986
pubmed:articleTitle
Murine IgA binding factors (IgA-BF) suppressing IgA production: characterization and target specificity of IgA-BF.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't