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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1 Pt 2
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pubmed:dateCreated |
1986-2-20
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pubmed:abstractText |
Permeability-surface area products of the capillary wall, PSc, and the myocyte sarcolemma, PSpc, for D-glucose and 2-deoxy-D-glucose were estimated via the multiple indicator-dilution technique in isolated blood-perfused dog and Tyrode-perfused rabbit hearts. Aortic bolus injections contained 131I-albumin (intravascular reference), two of three glucoses: L-glucose (an extracellular reference solute), D-glucose, and 2-deoxy-D-glucose. Outflow dilution curves were sampled for 1-2.5 min without recirculation. The long duration sampling allowed accurate evaluation of PSpc by fitting the dilution curves with a multiregional axially distributed capillary-interstitial fluid-cell model accounting for the heterogeneity of regional flows (measured using microspheres and total heart sectioning). With average blood flow of 1.3 ml . g-1 . min-1, in the dog hearts the PSc for D-glucose was 0.72 +/- 0.17 ml . g-1 . min-1 (mean +/- SD; n = 11), and PSpc was 0.57 +/- 0.15 ml . g-1 . min-1. In the rabbit hearts with perfusate flow of 2.0 ml . g-1 . min-1 (n = 6), PSc was 1.2 +/- 0.1 and PSpc was 0.4 +/- 0.1 ml . g-1 . min-1. PSc for 2-deoxy-D-glucose was about 4% higher than for D-glucose and L-glucose in both preparations. Relative to L-glucose, there was no measurable transendothelial transport of either dextroglucose, indicating that transcapillary transport was by passive diffusion, presumably via the clefts between cells. The technique allows repeated measurements of D-glucose uptake at intervals of a few minutes; it may therefore be used to assess changes in transport rates occurring over intervals of several minutes.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/HL-19135,
http://linkedlifedata.com/resource/pubmed/grant/HL-19139,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR001243-200014,
http://linkedlifedata.com/resource/pubmed/grant/R01 HL019139-24,
http://linkedlifedata.com/resource/pubmed/grant/RR-01243
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
250
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H29-42
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pubmed:dateRevised |
2011-9-22
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pubmed:meshHeading |
pubmed-meshheading:3510568-Animals,
pubmed-meshheading:3510568-Biological Transport,
pubmed-meshheading:3510568-Capillary Permeability,
pubmed-meshheading:3510568-Coronary Circulation,
pubmed-meshheading:3510568-Deoxy Sugars,
pubmed-meshheading:3510568-Deoxyglucose,
pubmed-meshheading:3510568-Dogs,
pubmed-meshheading:3510568-Glucose,
pubmed-meshheading:3510568-Indicator Dilution Techniques,
pubmed-meshheading:3510568-Models, Cardiovascular,
pubmed-meshheading:3510568-Myocardium,
pubmed-meshheading:3510568-Permeability,
pubmed-meshheading:3510568-Rabbits,
pubmed-meshheading:3510568-Sarcolemma
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pubmed:year |
1986
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pubmed:articleTitle |
Multiple tracer dilution estimates of D- and 2-deoxy-D-glucose uptake by the heart.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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