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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-5
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pubmed:dateCreated |
1989-6-12
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pubmed:abstractText |
Perfusion of isolated rat livers with ethanol at a concentration of 2 g/l (%o) resulted in a release of glutamate-pyruvate-transaminase (GPT) and sorbitol dehydrogenase (SDH) into the perfusate as markers of toxicity. Inhibition of alcohol dehydrogenase by 4-methylpyrazole or of aldehyde dehydrogenase by cyanamide totally abolished ethanol hepatotoxicity despite of a severalfold increase in acetaldehyde concentration in the perfusate. Addition of superoxide dismutase or catalase clearly suppressed the ethanol-induced release of GPT and SDH, suggesting that .O2- and H2O2 are involved in this process. Also, chelation of iron ions by means of desferrioxamine displayed a clear inhibitory action, suggesting the involvement of an iron-catalyzed Haber-Weiss-reaction leading to the formation of .OH radicals in the hepatotoxic response to ethanol. Our data suggest that during the metabolism of acetaldehyde primary reactive oxygen species (.O2-, H2O2) are produced which may interact to yield hydroxyl or .OH-like radicals, which possibly represent the hepatotoxic principle of ethanol.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alanine Transaminase,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanol,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/L-Iditol 2-Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Lactates,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvates
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pubmed:status |
MEDLINE
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pubmed:issn |
8755-0199
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19-26
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3508430-Alanine Transaminase,
pubmed-meshheading:3508430-Animals,
pubmed-meshheading:3508430-Ethanol,
pubmed-meshheading:3508430-Free Radicals,
pubmed-meshheading:3508430-L-Iditol 2-Dehydrogenase,
pubmed-meshheading:3508430-Lactates,
pubmed-meshheading:3508430-Liver,
pubmed-meshheading:3508430-Male,
pubmed-meshheading:3508430-Oxygen,
pubmed-meshheading:3508430-Perfusion,
pubmed-meshheading:3508430-Pyruvates,
pubmed-meshheading:3508430-Rats,
pubmed-meshheading:3508430-Rats, Inbred Strains
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pubmed:year |
1987
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pubmed:articleTitle |
Alcohol-induced hepatotoxicity: a role for oxygen free radicals.
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pubmed:affiliation |
Institute of Toxicology, Medical University of Lübeck, W.-Germany.
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pubmed:publicationType |
Journal Article,
In Vitro
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