Linked Life Data

3507556

Source:http://linkedlifedata.com/resource/pubmed/id/3507556

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-4-27
pubmed:abstractText
Virulent Y. enterocolitica strains restrict their growth at 37 degrees C, in rich medium deprived of calcium. This property, called calcium dependency, correlates with pathogenicity. It is conditioned by a 70 kb plasmid called pYV. The analysis of calcium independent (Cl) insertion mutants defined a 20 kb region called the calcium region. This region contains at least three transcription units called virA, B and C. In growth restriction conditions, Y. enterocolitica releases and inserts in its outer-membrane several pYV encoded proteins (POMPs). By transposition mutagenesis using a mini-Mu dlac element, we localized plasmid genes encoding POMPs of 84, 51, 30 and 25 kDa. These genes appear to be scattered on pYV, outside the calcium region and to constitute a regulon. Transcription of these yop genes increased by factors of 3.5 (yop84) to 200 (yop51) when temperature was shifted from 25 to 37 degrees C. On the contrary, calcium had only a moderate effect. Transcription was also dependent on the culture medium and on a trans-acting factor encoded by the calcium region. One Cl double mutant severely affected in transcription of yop51 defined a new locus called virF in the calcium region of pYV. Transcription of the yop genes was very poor in E. coli K12, even in the presence of a fully functional calcium region.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0882-4010
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
367-79
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Transcription of the yop regulon from Y. enterocolitica requires trans acting pYV and chromosomal genes.
pubmed:affiliation
Unité de Microbiologie, Université Catholique de Louvain, Brussels, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't